Protein Enrichment through Albumin Removal
Fast Results - Remove Albumin from Human Serum Samples in 30 minutes or less
Albumin removal tested and successfully removed from human, mouse, rat, sheep, goat and rabbit serum.
The BioMag® ProMax Albumin Removal Kit is supplied with particles and buffers for carrying out 25 separation reactions.
Figure 1 Panel A
SDS-PAGE analysis and Western Blot showing enrichment of lower abundance proteins and depletion of albumin.
Shows a silver stained SDS-PAGE gel. Lane 1, Molecular Weight Markers; Lane 2, untreated normal human serum; Lane 3, serum treated with ProMax Albumin Removal Particles. Both Lanes 2 and 3 were loaded with the same amount of protein. Panel B
Shows the depletion of albumin by Western Blot. Lane 1, normal serum; Lane 2, serum treated with the BioMag® ProMax Albumin Removal Kit; Lane 3, Molecular Weight Markers. Mouse anti-albumin was the primary antibody and the signal was visualized using an anti-mouse horseradish peroxidase conjugate and TMB as the chromagen. Both Lanes 1 and 2 were loaded with equal amounts of protein. Lane 2 shows that nearly all of the albumin has been depleted from the sample.
Based on patented BioMag® particle technology