GenomeWalker Kits provide a simple, PCR-based method for walking upstream or downstream in genomic DNA from a known sequence, such as an expressed sequence tag (EST). GenomeWalker allows you to take individual steps of up to 6 kb in genomic DNA using long-distance PCR. Walks can be extended simply by taking multiple steps, using new primers based on the sequence obtained in previous steps.
GenomeWalker Human Kit
The GenomeWalker Human Kit contains four premade libraries of adaptor-ligated, species-specific genomic DNA fragments. These libraries are constructed from highly pure genomic DNA of high molecular weight. The DNA is digested separately with four different restriction enzymes and ligated to the specially designed GenomeWalker Adaptor. Each kit contains the reagents necessary for up to 20 walks with each library, and 150 primary and 300 nested PCR reactions
GenomeWalker Universal Kit
The GenomeWalker Universal Kit allows you to construct GenomeWalker libraries from the genome of any species. The Universal Kit contains reagents sufficient to generate libraries from three different samples and to perform 80 walks.
For all kits, you must provide the set of gene-specific primers (GSP) and a mix of thermostable DNA polymerases suitable for long-distance PCR (LD PCR). We recommend the Advantage 2 Polymerase Mix or PCR Kit.
Note: The GenomeWalker Kits do not contain thermostable DNA polymerases for PCR.