Load SPRI-TE with Cartridges, Input Samples, Adapters and Labware
For each library to be constructed, insert one Piercing Tip and Sheath, two 1 mL Pipette Tips and Sheaths, one uncapped 2 mL Screw Cap Tube with Adapters, and one uncapped 2 mL Screw Cap Tube for the final library. If fewer than ten libraries are to be constructed during a run, align tips and tubes with the lane positions of the reagent cartridges in the Reagent Rack.
NOTE: Only use tips and tubes provided by Beckman Coulter.
For Each Library to be Constructed:
Insert a Piercing Tip and Sheath into row 1.
Insert a 1 mL Pipette Tip and Sheath into row 2.
Insert a 1 mL Pipette Tip and Sheath into row 3.
Aliquot 10 μL of adapters (50 μM) into a labeled, uncapped 2 mL Screw Cap Tube, and insert the tube into row 4.
Insert a labeled, uncapped 2 mL Screw Cap Tube into row 5. This tube will contain the fragment libraries at the conclusion of the run.