Spriworks System I - Products

By: Agencourt  06-Dec-2011

Step 3

Load SPRI-TE with Cartridges, Input Samples, Adapters and Labware

For each library to be constructed, insert one Piercing Tip and Sheath, two 1 mL Pipette Tips and Sheaths, one uncapped 2 mL Screw Cap Tube with Adapters, and one uncapped 2 mL Screw Cap Tube for the final library. If fewer than ten libraries are to be constructed during a run, align tips and tubes with the lane positions of the reagent cartridges in the Reagent Rack.
NOTE: Only use tips and tubes provided by Beckman Coulter.

For Each Library to be Constructed:

Insert a Piercing Tip and Sheath into row 1.

Insert a 1 mL Pipette Tip and Sheath into row 2.

Insert a 1 mL Pipette Tip and Sheath into row 3.

Aliquot 10 μL of adapters (50 μM) into a labeled, uncapped 2 mL Screw Cap Tube, and insert the tube into row 4.

Insert a labeled, uncapped 2 mL Screw Cap Tube into row 5. This tube will contain the fragment libraries at the conclusion of the run.


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